Cd8a-IRES-Luc-2A-EGFP

Nomenclature

C57BL/6Smoc-Cd8aem1(V5-IRES-luci-2A-EGFP)Smoc

Cat. NO.

NM-KI-18030

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
Cd8a

Model Description

A V5-IRES-luci-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knockin model with V5, luciferase and EGFP fluorescent protein.
Research Application:gene tracing

Validation Data

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_05.png

Figure 1  In the absence of any stimulation, the in vivo imaging system detected that Cd8aV5-IRES-luci-2A-EGFP/+ mice showed significant fluorescence.

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_06.png

Figure 2  In vivo imaging validation of MC38 tumors in Cd8aV5-IRES-luci-2A-EGFP/+ reporter mice treated or not with a PD-1 mAb. After 1 day of stimulation with PD1 antibody, the fluorescence was significantly enhanced.

Cd8aV5-IRES-luci-2A-EGFP-3.png

Figure 3  EGFP expression analysis in  PBMC of Cd8aV5-IRES-luci-2A-EGFP/+ mice by flow cytometry.


You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Learn more