Imaging Analysis


Service content: Analysis of bone density, trabecular bone and other parameters

Sample requirements: Live mice or local specimens of mice. The bone samples of mice, rabbits, and dogs should not exceed 10 cm in length.

Testing instruments: Micro-CT (eXplore Locus, GE Healthcare)

Principle: X-ray tomography and computer reconstruction are non-destructive 3D imaging techniques used to study the internal microstructure of a sample. The difference between micro-CT and conventional CT is that the resolution of micro-CT can reach the micron level.

Service cycle: Regular scan 25 min/time, whole-body in vivo scan 1 h/mouse (without tail)

Notice to customers: Do not decalcify fresh or fixed specimens used for scanning. Contrast-assisted imaging is required for tissues such as liver, blood vessels, and intestines.

microCT.JPG

Figure 1. Left panel: A screenshot of the skeletal image of an entire mouse; Right panel: Scan of lung tumor.

Principle: The luciferase reporter gene system is a reporter system that uses luciferin as a substrate to detect the activity of firefly luciferase. Luciferase can catalyze the oxidation of luciferin to produce oxyluciferin and this oxidation process emits bioluminescence. A fluorometer, also known as a luminometer or a liquid scintillation counter, can measure the bioluminescence released during the oxidation of luciferin.

Sample requirements: Fluorescently labeled mouse or tissue

Testing instruments and consumables: In vivo animal fluorescence imaging system (America/Roper Scientific), Luciferin

Service cycle: 1 business day. The service cycle of some fluorescence tracking experiments will be determined according to the experimental plan

Notice to customers: An appointment should be made at least 3 days in advance. It is recommended to collect organ or tissue samples on-site for imaging. For the in vivo imaging of mouse fluorescence, the target part should be shaved

ivis.JPG

Figure 1. In vivo imaging of live mice showing lung metastasis of luciferase-labeled tumor cells


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