hIL12B

The endogenous mouse Il12b gene was replaced by human IL12B gene.

Fig1. Detection of IL12B expression in thymus by RT-PCR. 

Wild type: only one band at 260 bp with primers F1/R1(mIl12b);

Heterozygous: one band at 260 bp with primers F1/R1(mIl12b) and one band at 260 bp with primers F2/R2(hIL12B); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig 2. Detection of hIL12B expression in plasma by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type. 

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

Fig 3. Detection of mIL12B expression in plasma by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type. 

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

Fig4. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.