Psoriasis Mouse Model


Establishment of Psoriasis Mouse Model

Methods

1. Healthy male BALB/c mice aged 7-8 weeks were selected for back shaving with an area of 2.5cm×2.5cm. 

2. Then the mice received commercially available IMQ cream (Sichuan Mingxin Pharmaceutical Co. LTD) on the shaved back for 5 consecutive days. The control group received the same dose of vaseline.

3. PASI (Psoriasis Area and Severity Index) and pathological changes were used to measure the severity and extent of psoriasis.

 

PASI scores can be calculated as follows:

Erythema, scaling, and thickening were scored independently on a scale from 0 to 4.

 

Thickening:

0No skin thickening. The flesh should be loose and unwrinkled.

1Visible skin puckering at the edge of the applied area.

2Visible skin puckering in all areas.

3The skin is further puckered.

4Based on the symptoms described above, the mice were losing weight or were in poor condition.

  

Scaling

0No skin flaking

1Minor dry spots

2Dry spots across a majority of the skin

3More and more dry spots

4Based on the symptoms described above, the mice were losing weight or were in poor condition.

 

Redness:

0Fleshy pink.

1Minor reddening

2Medium red

3Further red

4Based on the symptoms described above, the mice were losing weight or were in poor condition.

 

The cumulative score (erythema plus scaling plus thickening) served as a measure of the severity of inflammation (scale 0–12).

 

Results:

Mice with IMQ ointment showed erythema, scaly skin, and significantly thickened skin. HE staining results indicated that IMQ treatment resulted in hyperproliferative keratinocytes, a disturbed epidermal differentiation (parakeratosis), and the absence of a granular layer, admixed with lymphoid infiltrate and dilated capillaries in the upper dermis.

图片 3.png 

Fig 1. The picture of naïve and model mice at the end of experiment

图片 3_2.png 

Fig 2. Score and weight of naïve and model mice

(**, P<0.01, ***, P<0.0001)

 

图片 27.png 

Fig 3. HE staining from skin of the naïve and modelmiceat the end of experiment

对象 1.png

Fig 4. The thickness of skin of the naïve and model mice at the end of experiment

 (***, P<0.0001)


Conclusion

In conclusion, the IMQ-induced dermatitis mouse model shows many similarities with human psoriasis, which offered an ideal animal model for drug efficacy evaluation.


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