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Gene Summary

Gene Symbol

Model Description

The endogenous mouse Tnfrsf9 gene was replaced by human TNFRSF9(4-1BB) gene. While h4-1BB mice(Stock No.NM-HU-00099)have been pulled from shelves for some reasons.
Research Application:Immunotherapy,Cancer research,Drug screening

Validation Data


Figure 1. Expression of humanized CD137(4-1BB) in the activated spleen lymphocytes of heterozygous humanized 4-1BB mice is detected by FACS. 

The spleen lymphocytes of heterozygous humanized 4-1BB mice were activated by anti-CD3 and anti-CD28 for 48 hours, and then collected for staining. The expression of humanized 4-1BB was detected by FACS. The results showed that the active expression of humanized 4-1BB can be detected in both activated CD4+ and CD8+ T lymphocytes collected from heterozygous humanized 4-1BB mice. (Cooperation with CrownBio)

NM-HU-190077 h4-1BB-fig2.png

Figure 2  Analysis of human 4-1BB(CD137) expression in the spleen by FACS. The homozygous KI mouse expresses human 4-1BB on the CD8+ T cells.



Figure 3. In vivo validation of humanized 4-1BB mice. Humanized 4-1BB mice were inoculated with MC38 colon cancer cells, and were randomly assigned into different groups (n=6) when the tumors grew to a volume of 100 mm3. Human-specifc, 4-1BB antibody ( Urelumab or Utomilumab) was given every three days (Q3D) for two weeks (In collaboration with Crownbio).  

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