hCRBN

Fig1. Detection of CRBN expression in spleen, brain, liver, intestine and muscle tissues by RT-PCR. 

Wild type: only one band at 185 bp with primers F1/R1 (mCrbn);

Homozygous: one band at 262 bp with primers F2/R2 (hCRBN) and one band at 374 bp with primers F3/R3 (hCRBN);

Abbr. M, DNA marker; HO, homozygous;  WT, wild type.

Fig2. Expression characterization of hFCRN in homozygous hCRBN KI mice by Western blot.

Fig3. The degradation of GSPT1, CK1a and Myc by Lenalidomide and CC-885 in hCRBN knockin mice were determined by western blot.

The splenocytes derived from wild-type C57BL/6 (WT) and homozygous hCRBN KI (HO) mice were treated with 1% DMSO, Lenalidomide (10 nM, 100 nM, 1000 nM) and CC-885 (10 nM, 100 nM, 1000 nM) ,respectively, in RPMI-1640 cell culture medium for 18 hours ex vivo.