Cd19-IRES-DTRGFP

Nomenclature

C57BL/6Smoc-Cd19em2(IRES-DTREGFP)Smoc

Cat. NO.

NM-KI-190042

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
CD19

Model Description

A IRES-DTREGFP expression cassette was knocked into the Cd19 gene stop codon site.
Research Application:DTR mouse

Validation Data

Validation data of EGFP expression

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Fig1.  Validation EGFP expression in PBMC by FACS. (Cooperation with CrownBio)

Validation data of DTR function

Heterozygous Cd19-IRES-DTRGFP mice and C57BL/6 mice were inoculated with MC38 colon cancer cells. After the tumors grew to 100 mm3, the Cd19-IRES-DTRGFP mice were injected with Diphtheria toxin. 

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Fig2. The growth of MC38 tumors in Heterozygous Cd19-IRES-DTRGFP mice and C57BL/6 mice. (Cooperation with CrownBio)

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Fig3. The percent of CD19+ cell in peripheral blood of MC38 tumor-bearing Cd19-IRES-DTRGFP mice and C57BL/6 mice is detected by FACS. (Cooperation with CrownBio)

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Fig4. The percent of CD19+ cell in peripheral blood of MC38 tumor-bearing Cd19-IRES-DTRGFP mice and C57BL/6 mice is detected by FACS. (Cooperation with CrownBio)


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